Release notes for GTDB R04-RS89 Release date: June 17th, 2019 GTDB R04-RS89 contains 23,458 bacterial and 1,248 archaeal species clusters which span 145,904 genomes. MAJOR CHANGES from R03-RS86 --------------------------- - GTDB now uses average nucleotide identity to circumscribe species clusters - GTDB species clusters are defined by a single representative genome which is a genome assembled from the type strain where applicable - All genomes now have taxonomic assignments from the rank of domain to species - Archaeal reference tree is now inferred with IQ-Tree v1.6.9 under the PMSF model, a rapid approximation of the C10 mixture model (LG+C10+F+G), using FastTree v2.1.10 to infer an initial guide tree - Bacterial reference tree is now inferred with FastTree v2.1.10 under the WAG model - Reference trees now contain 1 genome per GTDB species cluster - Archaeal and bacterial reference trees are now inferred from filtered MSAs that span ~5,000 amino acids selected from the 122 archaeal and 120 bacterial previously used in full for phylogenetic inference - The taxonomy browser (gtdb.ecogenomic.org/tree) colour highlights type species and type strains MINOR UPDATES ------------- August 9, 2019: ar122_r89.tree was updated to contain bootstrap values and to be rooted on DPANN